Review




Structured Review

Merck & Co polybrene transfection reagent
Polybrene Transfection Reagent, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polybrene+transfection+reagent/genejuice+reagent+transfection/pm41922632-159-32-35
Average 86 stars, based on 1 article reviews
polybrene transfection reagent - by Bioz Stars, 2026-10
86/100 stars

Images

Related Articles

Infection:

Article Title: The functional role of Nudt2 in human triple negative breast cancer
Article Snippet: .. They were infected with either Nudt2 shRNA lent viral particles (#sc-60188-V; Santa Cruz Biotechnology, Almog, Israel) or control shRNA lentiviral particles (# sc-108080; Santa Cruz Biotechnology) using Polybrene transfection reagent (#TR-1003-G; Merck, Israel) reaching a final concentration of 8 μg/ml. ..

shRNA:

Article Title: The functional role of Nudt2 in human triple negative breast cancer
Article Snippet: .. They were infected with either Nudt2 shRNA lent viral particles (#sc-60188-V; Santa Cruz Biotechnology, Almog, Israel) or control shRNA lentiviral particles (# sc-108080; Santa Cruz Biotechnology) using Polybrene transfection reagent (#TR-1003-G; Merck, Israel) reaching a final concentration of 8 μg/ml. ..

Control:

Article Title: The functional role of Nudt2 in human triple negative breast cancer
Article Snippet: .. They were infected with either Nudt2 shRNA lent viral particles (#sc-60188-V; Santa Cruz Biotechnology, Almog, Israel) or control shRNA lentiviral particles (# sc-108080; Santa Cruz Biotechnology) using Polybrene transfection reagent (#TR-1003-G; Merck, Israel) reaching a final concentration of 8 μg/ml. ..

Transfection:

Article Title: The functional role of Nudt2 in human triple negative breast cancer
Article Snippet: .. They were infected with either Nudt2 shRNA lent viral particles (#sc-60188-V; Santa Cruz Biotechnology, Almog, Israel) or control shRNA lentiviral particles (# sc-108080; Santa Cruz Biotechnology) using Polybrene transfection reagent (#TR-1003-G; Merck, Israel) reaching a final concentration of 8 μg/ml. ..

Article Title: Preclinical evaluation of CRISPR-based therapies for Noonan syndrome caused by deep-intronic LZTR1 variants
Article Snippet: .. Lentiviral transduction of iPSC-CM cultures was performed in culture medium supplemented with 100 U/mL penicillin, 100 μg/mL streptomycin (Thermo Fisher Scientific), and 10 μg/mL Polybrene Transfection Reagent (Merck) and cells were transduced with 1.5 mL of the respective lentivirus. ..

Article Title: Astrocytic β-catenin signaling via TCF7L2 regulates synapse development and social behavior.
Article Snippet: .. After 24 h, in the presence of Polybrene Transfection Reagent (Merck Life Science, catalog no. TR-1003-G, 5 μg/ml), cells were transduced with a single lentiviral vector that expressed four transcription factors (Oct4, Klf4, Sox2, and cMyc) from a single transcript. .. After 48 h, transduced fibroblasts were split and cultured with a feeder layer of mitomycin C inactivated mouse embryonic fibroblasts (MEFs; Mitomycin C, Merck Life Science, catalog no. M5353, 10 μg/ml concentration) and cultured in iPSC medium (DMEM/F-12, Gibco, catalog no. 11320-074; 20% Knockout Serum Replacement, Gibco, catalog no. 10828010, 20% concentration; MEM Non-Essential Amino Acid Solution, Gibco, catalog no. 11140050, 1x; GlutaMAX, Thermo Scientific, catalog no. 35050087, 1x; penicillin-streptomycin, Gibco, catalog no. 15140148, 100 U/ml concentration; 2-mercaptoethanol, Gibco, catalog no. 31350010, 100 μM concentration; recombinant human bFGF protein, Alamone Labs, catalog no. F-170, 10 ng/ml concentration; sodium pyruvate, Gibco, catalog no. 11360070, 1mM concentration).

Article Title: Mutation-induced LZTR1 polymerization provokes cardiac pathology in recessive Noonan syndrome.
Article Snippet: Lentivirus was diluted in culture medium supplemented with 100 U/ml penicillin, 100 mg/mL streptomycin (Thermo Fisher Scientific), and 10 mg/mL Polybrene Transfection Reagent (Merck). .. Lentivirus was diluted in culture medium supplemented with 100 U/ml penicillin, 100 mg/mL streptomycin (Thermo Fisher Scientific), and 10 mg/mL Polybrene Transfection Reagent (Merck). .. For live-cell imaging, biosensor-transduced iPSC-CM cultures at day 60 of differentiation were treated with 100 nM MEK inhibitor trametinib (Selleck Chemicals), 100 nM JNK inhibitor JNK-IN-8 (Hycultec), or 1:1,000 DMSO (SigmaAldrich) for 60 min, before stimulation with 10% fetal bovine serum for another 60 min.

Article Title: Fine-tuning BACH2 dosage balances stemness and effector function to enhance antitumor T cell therapy.
Article Snippet: Primary mouse T cell transduction CD45.1+ OT-I splenocytes were activated for 24 h in complete RPMI media (RCM; Gibco) containing 100 IU ml−1 rhIL-2, 10 μg ml−1 anti-CD3 and 5 μg ml−1 anti-CD28 antibodies. .. Activated T cells were resuspended at 1 × 106 cells per ml in viral supernatant containing 100 IU ml−1 rhIL-2 and 8 μg ml−1 polybrene transfection reagent (Merck). ..

Article Title: Generation of two induced pluripotent stem cell lines (HIMRi006-A and HIMRi007-A) from Pompe patients with infantile and late disease onset.
Article Snippet: HDF were seeded at a cell density of 30,000 cells/cm2 on Matrigel®coated culture dishes (Corning®) in HDF growth media. .. On the following day, cells were transduced with 100 μl lentivirus concentrate per cm2 of culture area in the presence of 6 μg/ml polybrene transfection reagent (Merck, #TR-1003-G). .. Three days after transduction, hiPSC media (1 % Pen/Strep in StemFlexTM, ThermoFisher Scientific, # A3349401) was added to the culture medium at a 1:1 ratio, before switching to hiPSC media entirety on day five.

Article Title: A long-range enhancer at -52Kb drives expression of the COUP-TFII transcription factor in erythroid cells.
Article Snippet: .. Next, approximately 3x106 K562 cells stably expressing Cas9 (K562-Cas9) were seeded per well of a 6-well plate and transduced by spinfection (1000 rcf, 90 min) with lentiviral supernatants (diluted 1:2) supplemented with Polybrene Transfection Reagent (Merck, TR-1003-G). ..

Article Title: Fine-tuning BACH2 dosage balances stemness and effector function to enhance antitumor T cell therapy
Article Snippet: CD45.1 + OT-I splenocytes were activated for 24 h in complete RPMI media (RCM; Gibco) containing 100 IU ml −1 rhIL-2, 10 μg ml −1 anti-CD3 and 5 μg ml −1 anti-CD28 antibodies. .. Activated T cells were resuspended at 1 × 10 6 cells per ml in viral supernatant containing 100 IU ml −1 rhIL-2 and 8 μg ml −1 polybrene transfection reagent (Merck). ..

Concentration Assay:

Article Title: The functional role of Nudt2 in human triple negative breast cancer
Article Snippet: .. They were infected with either Nudt2 shRNA lent viral particles (#sc-60188-V; Santa Cruz Biotechnology, Almog, Israel) or control shRNA lentiviral particles (# sc-108080; Santa Cruz Biotechnology) using Polybrene transfection reagent (#TR-1003-G; Merck, Israel) reaching a final concentration of 8 μg/ml. ..

Transduction:

Article Title: Preclinical evaluation of CRISPR-based therapies for Noonan syndrome caused by deep-intronic LZTR1 variants
Article Snippet: .. Lentiviral transduction of iPSC-CM cultures was performed in culture medium supplemented with 100 U/mL penicillin, 100 μg/mL streptomycin (Thermo Fisher Scientific), and 10 μg/mL Polybrene Transfection Reagent (Merck) and cells were transduced with 1.5 mL of the respective lentivirus. ..

Article Title: Astrocytic β-catenin signaling via TCF7L2 regulates synapse development and social behavior.
Article Snippet: .. After 24 h, in the presence of Polybrene Transfection Reagent (Merck Life Science, catalog no. TR-1003-G, 5 μg/ml), cells were transduced with a single lentiviral vector that expressed four transcription factors (Oct4, Klf4, Sox2, and cMyc) from a single transcript. .. After 48 h, transduced fibroblasts were split and cultured with a feeder layer of mitomycin C inactivated mouse embryonic fibroblasts (MEFs; Mitomycin C, Merck Life Science, catalog no. M5353, 10 μg/ml concentration) and cultured in iPSC medium (DMEM/F-12, Gibco, catalog no. 11320-074; 20% Knockout Serum Replacement, Gibco, catalog no. 10828010, 20% concentration; MEM Non-Essential Amino Acid Solution, Gibco, catalog no. 11140050, 1x; GlutaMAX, Thermo Scientific, catalog no. 35050087, 1x; penicillin-streptomycin, Gibco, catalog no. 15140148, 100 U/ml concentration; 2-mercaptoethanol, Gibco, catalog no. 31350010, 100 μM concentration; recombinant human bFGF protein, Alamone Labs, catalog no. F-170, 10 ng/ml concentration; sodium pyruvate, Gibco, catalog no. 11360070, 1mM concentration).

Article Title: Generation of two induced pluripotent stem cell lines (HIMRi006-A and HIMRi007-A) from Pompe patients with infantile and late disease onset.
Article Snippet: HDF were seeded at a cell density of 30,000 cells/cm2 on Matrigel®coated culture dishes (Corning®) in HDF growth media. .. On the following day, cells were transduced with 100 μl lentivirus concentrate per cm2 of culture area in the presence of 6 μg/ml polybrene transfection reagent (Merck, #TR-1003-G). .. Three days after transduction, hiPSC media (1 % Pen/Strep in StemFlexTM, ThermoFisher Scientific, # A3349401) was added to the culture medium at a 1:1 ratio, before switching to hiPSC media entirety on day five.

Plasmid Preparation:

Article Title: Astrocytic β-catenin signaling via TCF7L2 regulates synapse development and social behavior.
Article Snippet: .. After 24 h, in the presence of Polybrene Transfection Reagent (Merck Life Science, catalog no. TR-1003-G, 5 μg/ml), cells were transduced with a single lentiviral vector that expressed four transcription factors (Oct4, Klf4, Sox2, and cMyc) from a single transcript. .. After 48 h, transduced fibroblasts were split and cultured with a feeder layer of mitomycin C inactivated mouse embryonic fibroblasts (MEFs; Mitomycin C, Merck Life Science, catalog no. M5353, 10 μg/ml concentration) and cultured in iPSC medium (DMEM/F-12, Gibco, catalog no. 11320-074; 20% Knockout Serum Replacement, Gibco, catalog no. 10828010, 20% concentration; MEM Non-Essential Amino Acid Solution, Gibco, catalog no. 11140050, 1x; GlutaMAX, Thermo Scientific, catalog no. 35050087, 1x; penicillin-streptomycin, Gibco, catalog no. 15140148, 100 U/ml concentration; 2-mercaptoethanol, Gibco, catalog no. 31350010, 100 μM concentration; recombinant human bFGF protein, Alamone Labs, catalog no. F-170, 10 ng/ml concentration; sodium pyruvate, Gibco, catalog no. 11360070, 1mM concentration).

Stable Transfection:

Article Title: A long-range enhancer at -52Kb drives expression of the COUP-TFII transcription factor in erythroid cells.
Article Snippet: .. Next, approximately 3x106 K562 cells stably expressing Cas9 (K562-Cas9) were seeded per well of a 6-well plate and transduced by spinfection (1000 rcf, 90 min) with lentiviral supernatants (diluted 1:2) supplemented with Polybrene Transfection Reagent (Merck, TR-1003-G). ..

Expressing:

Article Title: A long-range enhancer at -52Kb drives expression of the COUP-TFII transcription factor in erythroid cells.
Article Snippet: .. Next, approximately 3x106 K562 cells stably expressing Cas9 (K562-Cas9) were seeded per well of a 6-well plate and transduced by spinfection (1000 rcf, 90 min) with lentiviral supernatants (diluted 1:2) supplemented with Polybrene Transfection Reagent (Merck, TR-1003-G). ..



Similar Products

94
TargetMol polybrene transfection reagent
Polybrene Transfection Reagent, supplied by TargetMol, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polybrene+transfection+reagent/Hexadimethrine+bromide/pmc12548452-225-5-20
Average 94 stars, based on 1 article reviews
polybrene transfection reagent - by Bioz Stars, 2026-10
94/100 stars
  Buy from Supplier

97
MedChemExpress polybrene transfection reagent
Polybrene Transfection Reagent, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polybrene+transfection+reagent/Hexadimethrine+bromide/pmc12548452-225-5-17
Average 97 stars, based on 1 article reviews
polybrene transfection reagent - by Bioz Stars, 2026-10
97/100 stars
  Buy from Supplier

86
Merck & Co polybrene transfection reagent
Polybrene Transfection Reagent, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polybrene+transfection+reagent/genejuice+reagent+transfection/pm41922632-159-32-35
Average 86 stars, based on 1 article reviews
polybrene transfection reagent - by Bioz Stars, 2026-10
86/100 stars
  Buy from Supplier

86
Merck & Co polybrene infection transfection reagent
Polybrene Infection Transfection Reagent, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polybrene+transfection+reagent/genejuice+reagent+transfection/10__1158_slash_1535___7163__mct___25___0005-78-14-17
Average 86 stars, based on 1 article reviews
polybrene infection transfection reagent - by Bioz Stars, 2026-10
86/100 stars
  Buy from Supplier

97
Beijing Solarbio Science polybrene transfection reagent
Polybrene Transfection Reagent, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polybrene+transfection+reagent/Polybrene/pm41310183-227-10-13
Average 97 stars, based on 1 article reviews
polybrene transfection reagent - by Bioz Stars, 2026-10
97/100 stars
  Buy from Supplier

Image Search Results